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KMID : 0644120140270010081
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Journal of Oriental Gynecology
2014 Volume.27 No. 1 p.81 ~ p.100
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In Vitro Anti-bacterial and Anti-inflammatory Effects of Six Types of Herb Aqueous Extracts
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Jang Se-Ran
Kim Dong-Chul
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Abstract
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Objectives: The object of this study was to observe the in vitro anti-bacterial and anti-inflammatory effects of six single aqueous herbal extracts-Quisqualis Fructus (QuF), Meliae Cortex (MeC), Arecae Semen (ArS), Crassirhizomae Rhizoma (CrR),
Ulmi Pasta Semen(UlS), Torreyae Semen(ToS)- against Staphylococcus aureus (S. aureus) and Lipopolysaccharide(LPS)-activated Raw 264.7 cells.
Methods: Anti-bacterial activities against S. aureus of aqueous extracts of QuF, MeC, ArS, CrR, UlS and ToS were detected using standard agar microdilution
methods. In addition, the effects on the cell viability, prostaglandin E2 (PGE2), nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 productions of LPS activated Raw 264.7 cells were detected. The anti-bacterial and anti-inflammatory effects were respectively compared with lincomycin and piroxicam.
Results: Minimal Inhibition Concentration (MIC) of aqueous extracts of QuF, MeC, ArS, CrR, UlS and ToS against S. aureus was respectively detected 5.625±4.075
(3.125~12.500), 0.332±0.273 (0.098~0.782), 1.094±0.428 (0.782~1.563), 2.969±2.096 (0.782~6.250), 9.375±4.419 (3.125~12.500)>25 mg/ml. MIC of lincomycin was detected as 0.469±0.297 (0.195~0.782) μg/ml at same conditions. In addition, ED50 against LPS induced cell viabilities and cytokine releases of QuF, MeC, ArS, CrR, UlS
and ToS was as follows - Cell viability: 66.370, 2.908, 1.747, 259.553, 18.150 and 34.160 mg/ml; NO production: 389.486, 0.294, 0.138, 523.060, 45.363 and 49.327 mg/ml; PGE2 production: 114.271, 0.223, 0.046, 243.078, 8.829 and 28.947 mg/ml; TNF-α production: 406.288, 0.343, 0.123, 9404.227, 125.406 and 140.775 mg/ml; IL-1β production: 117.178, 0.135, 0.019, 237.451, 7.923 and 19.418 mg/ml; IL-6 production: 31.261, 0.105, 0.055, 128.434, 2.290 and 3.745 mg/ml. ED50 of piroxicam against LPS-induced cell viabilities, NO, PGE2, TNF-α, IL-1β and IL-6 were detected as 35.179, 6.552, 1.162, 7.273, 7.101 and 5.044 μg/ml, respectively at same conditions.
Conclusions: All six single aqueous herbal extracts showed anti-bacterial effects against S. aureus, in the order of MeC, ArS, CrR, QuF and UlS aqueous extracts except for ToS; they did not showed any anti-bacterial effects (MIC>25 mg/ml). They also showed anti inflammatory effects against LPS-activated Raw 264.7 cells in the order
of ArS, MeC, UlS, ToS, QuF and CrR aqueous extracts. It means that the ArS and MeC will be showed favorable potent anti-bacterial and related anti-inflammatory effects.
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KEYWORD
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Quisqualis Fructus, Meliae Cortex, Arecae Semen, Crassirhizomae Rhizoma, Ulmi pasta Semen, Torreyae Semen, Staphylococcus Aureus
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